Download Cell and Tissue Culture Models in Dermatological Research by M. Bachmann, W. E. G. Müller (auth.), Dr. August Bernd, PDF

By M. Bachmann, W. E. G. Müller (auth.), Dr. August Bernd, Professor Dr. Jürgen Bereiter-Hahn, Priv.-Doz. Dr. Frank Hevert, Professor Dr. H. Holzmann (eds.)

In this quantity the effect of phone tradition versions on dermatological examine is mentioned by means of scientists from drugs, body structure, biology, pharmacology,and pharmacy. The ebook contains the subsequent themes: orthology and pathology of the surface, differentiation of keratinocytes and fibroblasts, difficulties of the cultivation of melanocytes, biochemistry of pigment metabolism, types for wound therapeutic and tissue renewal, fibroblast functionality and metabolism of collagen, types for the research of aging in addition to versions for pharmacological and toxicologial exams. therefore, a large arch is spanned, from uncomplicated issues of cultivation and characterization of mobilephone cultures to examples of software. Dermatologists in addition to mobilephone biologists will take advantage of this publication.

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4H, griseofulvin treatment induced the cell-surface staining and strongly enhanced the amount of La protein deposited to the extracellular matrix. Similar alterations were also observed for mercury chloride [5], which is known to cause autoantibody production in mice. Such conditions could convert nonpathogenic to pathogenic antibodies. Conclusions Taken together, our cell culture system allows us to test if sera contain pathogenic ANAs and why these ANAs are or become pathogenic. In addition, our cell culture system allows us to test whether a drug induces or protects against autoimmune reactions: Pretreatment of cultured cells with chloroquine - a drug which is used to treat autoimmune patients - followed by a treatment with griseofulvin protected the cells against antibody binding (not shown).

Bernd et al. No differences were induced in fibroblasts from adults. Also with skin fibroblasts of an adult patient with psoriasis, no differences between the psoriasis and control sera were found. However, the cell growth was stimulated more by the sera of both collectives than by the purchased AB pool serum. As a example for established cell lines, the results with HaCaT cells are given. Here, too, there was no difference between the two sera concerning the mitogenic effect (Fig. 3). In a further experiment the psoriasis and the control sera each were pooled and the serum content of the culture medium was varied.

D. Falke for his support during herpesvirus studies. Special thanks are due to graduate students K. Pfeifer, S. H. Chang, B. Diehl-Seifert, T. Zaubitzer, C. Selenka, M. Pretzlaff, and T. Metzger. References 1. Bachmann M, SchrOder HC, Falke D, Muller WEG (1988) Alteration of the intracel- 2. 3. 4. 5. 6. 7. lular localization of the La protein compared with the localization of U snRNPs. Cell Bioi Int Rep 12:765-789 Bachmann M, Pfeifer K, Schroder HC, Muller WEG (1990) Charakterization of the auto antigen La as a nucleic acid-dependent ATPase/dA TPase with melting properties.

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